Technologies

• Isolation and characterization of MSC from bone marrow and adipose tissue
• Ex vivo amplification, introducing both standard and innovative culture conditions: -different media and proprietary media both for expansion and differentiation; -use of different culture supports (plates, flasks, bags, matrices);
• Immunophenotyping and sorting by FACS (FACSAria III, Becton Dickinson) or MACS Cell Separation (Miltenyi Biotec)
• Isolation and characterization by FACS and ZetaView technology of Micro, Nano Vesicles and Exosomes from cell lines and primary cells from patients, including either cancer and stem cells
• Time-lapse microscopy (Zeiss Axiovert 200M)
• Proliferation and migration assays (Cell Proliferation Assay by FACS analysis & BrdU, CFSE or propidium iodide staining; 96-well plate proliferation assays: MTS assay, fluorescent assay, luminescent cell viability assay; transwell systems, time lapse microscopy)
• Cytotoxicity assays both in vitro (FACS analyses: detection and quantification of apoptotic/necrotic cells by Supervital Propidium Iodine, Annexin V, activated Caspase3-8 staining; 51Cr release by Microbeta 2 Perkin Elmer;  Cell Viability Assay on the GloMax® Discover System-Pormega; time lapse microscopy)
• Plasmid vector construction for cell gene-modification and protein expression (FACS analyses)
• Gene manipulation of mesenchymal stromal/stem cells by viral vectors
• Western Blotting and in-cell Western by LI-COR Odissey Infrared Imaging System
• Multiparametric histological analyses and microscopy
• Early clinical implementation and regulatory requirement